技術(shù)服務 犬單克隆抗體制備

服務項目

(Service Items)

周期（周）

Time(weeks)

多肽

peptide

小分子

Molecular

磷酸基/甲基/乙基化多肽

Phosphate/Methyl/acetyl-specific

多肽設計與合成

Peptide design   & synthesis

多肽設計

Peptide design

~2

可選，免費

多肽合成

Peptide   synthesis

√

√

小分子或多肽偶聯(lián)

conjugation

√

√

√

免疫階段

Immunization

免疫比格犬1只

Immunized 1 dog

~8

√

√

√

ELISA檢測

ElISA detection

√

√

√

cDNA制備

cDNA preparation

逆轉(zhuǎn)錄制備cDNA文庫

prepare cDNA   library by RT-PCR

~1

√

√

√

cDNA 文庫質(zhì)檢

cDNA library QC   check

√

√

√

構(gòu)建Fab抗體庫

Fab library   construction

VH與VL基因獲得

obtained VH   and VL genes

10~15

√

√

√

構(gòu)建初級抗體庫

primary antibody   library construction

√

√

√

構(gòu)建Fab抗體庫

Fab library   construction

√

√

√

噬菌體篩選

phage screening

噬菌體顆粒包裝

phage particles   package

10~15

√

√

√

磁珠親和篩選

magnetic beads   affinity screening

√

√

√

陽性克隆回收基因測序

Recover positive   clones and DNA sequencing

√

√

√

抗體表達

Antibody   expression

特異性雙鏈基因合成與Fc基因橋接

synthetic of   target phage gene and Fc gene

~5

√

√

√

構(gòu)建真核表達載體

Eukaryotic   system expression plasmids construction

√

√

√

真核表達

Eukaryotic   system expression

√

√

√

穩(wěn)轉(zhuǎn)細胞系構(gòu)建

Stably transfected   cell line construction

純化與檢測

Purification and   detection

標簽純化

Tag affinity   purification

~1

√

√

√

特異性親和純化

Antigen specific   affinity purification

√

√

√

總周期（月）

Total lead   time(months)

7~9